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Correlation between methods were checked using linear best fit lines of log2 expression values for samples measured by RT-qPCR against microarray (using the microarray probe corresponding to the contig used for primer design).
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The presented methods were checked by the experiment and analytically.
Linearity of methods was checked within the 0 50 ppm.
The linearity of the method was checked by measuring six concentrations of T6P between 80 nM and 80 μM.
The proposed method is checked by numerical simulation.
Performance of the method is checked through numerical examples.
The convergence of this method was checked by error calculation.
The accuracy of the method was checked by recovery studies.
The method was checked against experimental data in the literature and compared with other prediction methods.
The performance of the method is checked for a wide variation of damage positions and extents.
Convergence of the method is checked using various Chebyshev polynomial terms.
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