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a Read abundance ratios between methods were calculated using the average read abundance in each method.
Agreements between methods were calculated using the Kappa test for staining intensities (Tables S2 and 4), and OCCC for positive tumor ratios (Table 4).
Correlations between methods were calculated both before and after SSMI scaling to demonstrate the effectiveness of these scaling parameters on absolute expression levels in other tissues.
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In addition to the Bland-Altman plots, the reproducibility score (RC), defined by 1.96*SD of the difference between methods was calculated, RC=1.96cdot sqrt{frac{1}{N}sum_{i=1}^N{Logan}}-{DVR}_i^{2mathrm{TCM}}-frac{1}{mathrm{TCM}}-frac{1}{N}sum_{j=1}^N{DVR}_j^{mathrm{Logan}}-{DVR}_j^{2mathrm{TCM}}right)}^2} (12 where N is the number of ROIs.
To avoid a systematical calculation error in the case of repeated measurements per subject, the bias between methods was calculated according to a modified statistical approach by Bland and Altman for unequal numbers of replicates [ 22].
Variation between duplicates and between counting methods were calculated to be = 2.5% In conjunction with the spinner flasks, Cytodex 3 microcarriers (GE Healthcare, Piscataway, NJ) were used for the cell lines studied [ 13].
The relative sensitivity (se), the relative specificity (sp) and the level of agreement (kappa; κ) between the isolation methods were calculated using Win Episcope 2.0.
All EBV were standardized to an additive genetic standard deviation of 12 and 100 for the mean of cohorts from birth year 18 to 22. Spearman rank correlations between TMI of different methods were calculated using SAS 9.2.
Significant differences in the detection of ten predefined findings between 2D and 3D imaging methods were calculated.
Average costs of all three methods were calculated between 468 and 470 € per admission.
Simpson's Index of Diversity and adjusted Wallace coefficients between the HRM10SNP and iPLEX20SNP methods were calculated using these 470 isolates (Table 4).
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