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Statistically significant differences (P < 0.05) between means of two groups were determined by Student's t test, while comparisons of more than two groups were performed by one-way ANOVA and Bonferroni post hoc analyses.
We used Student's t-test for significance of differences between means of two groups and one-way analysis of variance (ANOVA) for more than two groups.
Comparisons between means of two independent groups were carried put using a pair-wise t-test.
Comparisons between means of two groups were performed with the Mann-Whitney test.
The differences between means of two groups were analyzed by a two-tailed unpaired Student's t-test.
Differences between means of two groups were tested using a t-test/as well as Mann whitney test, with significance P < 0.05.
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The fold-change analysis was than applied to identify significant probes at each time point with at least 2-fold differential expression between means of three experiments in iTregs or Teffs and the respective CD25- cell population ("iTreg-" and "Teff-regulated probes", respectively).
Estimated parameters: variation of mean square (VMS), difference between mean of four maximum and mean of four minimum values (R4), power spectral density for frequency of 50 250 Hz (P1) and 250 450 Hz (P2) were analyzed.
Analysis of variance (ANOVA) test was used for comparison between means of the two groups.
However, statistically significant differences between means of the two others were reported (Table 3, part B).
Difference between means of the two types of spatial centres projected on the axis of highest variability was compared with the value obtained for real alignment.
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