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Our results on transcript abundance of pheromone precursor genes showed that transcript levels of hpp1 in the absence of a mating partner are higher in MAT1-2 and those of ppg1 are higher in MAT1-1, albeithehe difference between mating types is more extensive for hpp1 (Fig. 5A and B).
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Conjugation between haploid cells of opposite mating types is a prerequisite for infection, with the mating system tending strongly toward selfing and automixis [ 46- 48].
Using mating crosses, mating types were assigned to ten isolates of the heterothallic fungal species Ophiostoma quercus.
For mating, cells of two different mating types were mixed and allowed to conjugate [ 18].
For mating, cells of two different mating types were mixed and allowed to conjugate.
Both mating types were retained (FY5216: h + or FY5217: h −).
In heterothallic filamentous ascomycetes mating between cells of opposite mating type is accomplished by cells designated as male and female.
For example, white and opaque cells differ markedly in their ability to mate: mating between opaque cells of the opposite mating type is at least 106 times more efficient than mating between white cells [7].
Only matings between individuals of different mating type are allowed.
Nevertheless, reproduction between individuals of the same mating type was shown to allow recombination to occur [ 45].
Crosses between each wild-type strain and the reference strains of opposite mating type were used as positive controls for comparison with evolved strains.
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