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To examine the significance of the correlations between mappability and transcript length, expression levels, or evolutionary conservation (Figure 2C E), we separately each of the following genomic regions: the 200 intronic nt upstream of exons, the first 100 nt of exons, the terminal 100 nt of exons, and the 200 intronic nt downstream of exons.
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Uren and colleagues [ 90] directly modeled the background distribution of read counts and account for mappability and transcript abundance (and, optionally, data from a contrasting experiment for comparison of differential binding).
We observed a positive correlation between mappability and conservation with ∼10% differences in mappability between the most conserved and least conserved exons (Kruskal-Wallis rank sum test, P∼0) (Fig. 2D).
There are significant differences between your grade report and transcript.
Both mappability and control genome normalization reduce the total number and length of copy number regions.
A detailed comparison between the PolyA-seq [ 27] and PA-seq (this study) in mappability and identification of PA sites can be found in Additional file 15 and Additional file 16, respectively.
Accuracy of allelic abundance was increased further by excluding differentiating sites within sequence reads that could not be aligned uniquely within the genome (imperfect mappability) and reads that overlapped one or more insertions or deletions (indels) between alleles.
BS-Seeker2 has the similar mappability and mapping accuracy with Bismark and BSMAP on simulation data.
For the error-free data, BS-Seeker2 has the highest mappability and 100% accuracy.
The normalization methodologies evaluated in FREEC are: GC content, mappability and control genome.
Our mappability and GC-content tracks are based on the reference human genome version hg19.
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