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MECs are localized between luminal epithelial cells and the stroma, which ideally position them to communicate with both compartments.
Additionally, we have observed LRECs in basal epithelial positions defined as residing between luminal epithelial cells and myoepithelial cells.
Microarray gene signatures identified a comprehensive list of 907 and 955 transcripts whose expression differed between luminal epithelial cells and myoepithelial cells, respectively.
Consistent with previous reports, immunofluorescence revealed a similar distribution of Cx26, Cx32 and Cx30 gap junction plaques between luminal epithelial cells in Cx43I130T/+ mice compared with controls [ 20, 21].
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A clear distinction between luminal-epithelial cells and myoepithelial cells in TDLUs is frequently difficult to make on conventionally stained slides.
Myoepithelial cells synthesize the basement membrane and act as an active structural barrier between the luminal epithelial cells and the surrounding intra-lobular stroma [8].
Chris Jones (Institute of Cancer Research, London, UK) presented an elegant array study analyzing the differences between normal luminal epithelial cells and myoepithelial cells (abstract 20 [ 1]).
The DTET comprises 8,051 transcripts that show a significant difference in abundance between normal luminal epithelial cells and primary malignant epithelial cells, and 640 of these transcripts belong to the family of transcription factors [ 12].
These data suggest that NTN1, signaling though NEO1, maintains the integrity of the end bud by mediating contacts between cap and luminal epithelial cells, and these contacts are required for robust forward growth of the mammary tree.
Taken together, these results suggest that multiple pathways regulate adhesion between cap and luminal epithelial cells, and that disrupting the integrity of these layers severely compromises forward growth of the mammary tree.
Myoepithelial cells are ectodermally derived, as are luminal epithelial cells, and they rest between the basal side of the luminal cells and the basement membrane that they manufacture themselves [ 22].
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