Sequence contamination between bar-coded libraries were identified and removed when their sequence identities were over 98%, but their abundance of reads were > 50 fold between libraries.
Comparison between the data set presented here and the previously published cDNA library revealed that only 53 out of 623 total genes from the LCM library were identified as significantly expressed in the liver stage microarray analysis presented here.
Thus, the difference between our duplicons and duplicon library is probably due to the fact that our duplicons were annotated based on CNVs, whereas duplicons in the library were identified solely based on SDs.
Only 8% of the library was identified.
Redundancy within the subtracted library was identified by BLASTN analysis of the library against itself.
A total of 53,613,739 unique sRNA sequences including ones with SNPs between the parents were identified by combining all the reads from 104 libraries (Table 1).
From the soma library 91,888 tags were identified.
Studies published between 1990 and 2007 were identified through a library search of databases and an examination of reference lists of relevant publications.
Totally 11 primary metabolites significantly varied between the two extracts were identified based on MS fragmentation, library, and standards.
