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Hence we would expect there to be a positive correlation between intron size and gene expression levels.
It has thus been suggested that a relationship between intron size and divergence might only be expected for first introns [ 1].
The plant Arabidopsis thaliana has a compact genome and generally small introns [ 18], consistent with the proposed correlation between intron size and genome size [ 19, 20].
Authors' response: We consider that our hypothesis may provide some insight about the correlation between intron size and gene expression levels.
In plants and yeasts, most studies revealed a positive correlation between intron size and expression level (Juneau et al. 2006; Ren et al. 2006; Li et al. 2007; Jeffares et al. 2008).
Dr Igor B Rogozin, NCBI/NLM/NIH, USA (nominated by Dr I King Jordan) The paper discusses various issues related to the positive correlation between intron size and gene expression which is observed in unicellular organisms and some multicellular organisms.
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We calculated the correlation between the amplifiability and the genetic distance among species, and the correlation between the intron size and the genetic distance in order to address this question.
They also observed the tendency towards short or no introns in GC-rich genes and identified a correlation between GC content, intron size and location among homologs across species.
Analyses done on animal models however have reported a more diverse pattern when investigating relationships between expression levels and intron size and density.
The Pearson correlation coefficient between the difference in intron size and the genetic distance of every taxon-pair was estimated using the SAS program (SAS Institute Inc., Cary, NC, USA).
To test the correlation between two traits, such as intron size and genome size, we constructed a simple regression model y i = α + βx i + ε i, where y i is the dependent variable and x i is the independent variable.
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