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Genetic distances between haplotypes were based on the Kimura 2-parameter model.
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Additional amovas of mtDNA haplotypes were based on an FST analog statistic (ΦST) that accounts for both haplotype-frequency differences between samples and sequence divergences between haplotypes.
Genealogical relationships between haplotypes were estimated based on local genealogies.
The number of haplotypes was estimated and pairwise genetic distances between haplotypes were calculated based on the number of nucleotide differences using MEGA version 3.1 [ 25].
First, alternative connections between haplotypes were broken following a series of rules based on coalescence theory [ 90].
The differences (convergence and divergence values) between haplotypes were proved by these observed SNPs.
For AMOVA, computations were based on haplotype frequencies.
Some of our results were based on a haplotype approach.
Haplotypes represented are based on 683 base pairs of the mtDNA control region.
The method considers only the potential sharing of haplotypes between individuals and is based on transforming the graph of potential haplotype sharing into a line graph using a minimum number of edge and vertex deletions.
The relationship between haplotypes is portrayed in Fig. 2.
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