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Comparisons between groups were undertaken using unpaired Student's t-test.
Comparisons between groups were undertaken using an unpaired Student's t-test.
Analyses of continuous, normally distributed variables within and between groups were undertaken using the appropriate Student's t test.
The comparisons of demographics between groups were undertaken by χ-tests for categorical variables and Kruskal Wallis or t-tests for continuous variables as appropriate.
Comparisons between groups were undertaken using Chi square analysis to measure association for age, gender, marital status, dependents, rural origin, job satisfaction and intention to leave.
All statistical comparisons of metabolite concentrations and histological features between groups were undertaken in SPSS version 22 (IBM, Armonk, NY, USA) using the nonparametric Kruskall Wallis test with significance set at P⩽0.05.
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Comparison between groups was undertaken with independent sample t-tests or non parametric tests where appropriate, with p < 0.05 being considered significant.
The variance in the difference between each primary or coprimary outcome measure between groups was undertaken on an intention-to-treat basis using independent t test and Mann-Whitney U test.
Descriptive data analysis and comparisons between groups was undertaken using SPSS (Statistical Package for the Social Sciences Version 14.0, SPSS Inc., Chicago, Illinios, U.S.A .. Descriptive statistics were used to summarise the data and a t test was used to compare the difference between genders.
Comparisons of continuous variables between two groups were undertaken using the Mann–Whitney U test, and the Kruskal Wallis test was used to compare multiple groups.
Pathway analyses for genes that were significantly different between the two groups were undertaken with the use of GenMapp software, and identified pathways that are frequently reported to be perturbed during carcinogenesis (Tables 2a and 3a).
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