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One possibility is that MYC is expressed at a high level across all tumours irrespective of the copy number status and hence is not different between groups of tumours that show a gain and those that do not.
GPS scores were then compared between groups of tumours classified according to the tumour stage or relapse status.
Testing for significant differences in the GPS expression or immunohistochemical indices between groups of tumours was carried out with SPSS 15.0.0 (SPSS Inc., Chicago, IL, USA).
No significant differences in the expression of the examined molecules were detected between groups of tumours with different histological grade or type (data not shown).
We did not find any significant differences between groups of tumours from carriers with different mutations or when comparing tumours from the carriers of the mutations at the 5' end or the 3' end of the gene.
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Two-tailed Fisher's exact test was used for pair-wise comparison of markers to detect differences between two groups of tumours.
To determine whether CRC proliferative activity inversely correlates with increasing metastatic potential, we compared GPS scores between three groups of tumours.
The χ-test and Fisher's exact test were employed for the analysis of the homogeneity of the distribution of the expression of imunochistochemical markers, tumour grade and subtypes between the groups of tumours using SPSS 13.0 (SPSS, Chicago, IL, USA) and R/Bioconductor.
All features were highly comparable between the two groups of tumours.
Our study identified a large number of potentially important methylation events that could contribute to the tumour's pathogenesis, among which the methylation of several genes correlated well with their expression levels between the two groups of tumours.
The data generated from the microarray study was analysed using t-test analysis, resulting in a set of 51 genes that differed in average expression between the two groups of tumours with p < 0.001 (table 2).
More suggestions(16)
between subgroups of tumours
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