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Comparisons of measurements between groups of mice were made using Student's unpaired T test with an alpha level of 0.05 considered significant.
Statistical differences between groups of mice were evaluated by the Student-t or Mann-Whitney tests.
Statistical differences in tumor sizes between groups of mice were determined by one-way ANHER.
At baseline, no differences in bilirubin levels between groups of mice were detected (Table 1; values not shown).
Statistical differences between groups of mice used in this study were evaluated by the Student's t test.
Quantitative cultures of lung and brain were used to compare tissue burdens between groups of mice sacrificed on day 4, 7 and 14 following inoculation.
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The Mann-Whitney test was used throughout to test for significance in the ELISPOT and ELISA data and the tissue load of MAP between groups of individual mice.
A, All comparisons are between groups of AD Tg mice.
Abbreviations: Tg=transgenic, mos=months, d=day, Gnx=gonadectomy, SAB=spontaneous alternating behavior (thought to reflect working memory), IP=intraperitoneal, Veh=Vehicle, DHT=dihydrotestosterone. A, All comparisons are between groups of AD Tg mice.
Data acquired for each mouse over the course of the study were normalized with respect to baseline values for each mouse to minimize biological variability between different mice, thus allowing us to assess longitudinal changes between different groups of mice.
Moreover, at day 2 after immunization, significant differences in mean antibody titers could be detected between the groups of mice receiving either vaccine co-administered with the RNA and the control group, unlike those immunized with the vaccine alone.
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