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The anti-ganglioside reactivity also showed a differential pattern between groups of chickens.
In GB11 challenged chickens, the IgG antibody response against GB11 protein was slightly stronger than against Pen HS 3 protein (Fig. 1A), but generally, there was considerable cross-reactivity between groups of chickens.
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The first possible explanation is the presence of genetically based differences between the groups of chickens in handling and processing of bacterial antigens by the immune system [19].
Furthermore, the qualitative and quantitative differences between and within groups of chickens emphasize host-dependent factors in the generation of anti-ganglioside antibodies following C. jejuni inoculation and make the chicken infection model suited for studying the pathogenesis of GBS following C. jejuni infection.
CA3 is also differentially expressed between the two groups of chickens in the present study, and is upregulated in affected birds.
(ii) Is there any difference in anti-ganglioside response either between or within genetically different groups of chickens.
The gene expression data were compared in order to identify the differentially expressed genes between Medicated and Non-medicated groups of chickens at each time point before and after clostridial challenge, including D0, D1, D2, and D4 PI.
In Fig. 2, we explored the proportion of variants shared by different groups of chickens, viz., between commercial (consisting of WEL and BEL) and experimental lines (including inbred and RI-J), and between WEL and BEL groups.
Experimental set-up for the three groups of chickens involved in the study.
In parallel, two other groups of chickens were treated intranasally (i.n).n
Groups of chickens were vaccinated with rNDV, rNDV-HA, rNDV-HAF, or were left unvaccinated.
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