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While expression of p16INK4a is highly dynamic in PBTL with age, differences between genotypes were significant at all ages (Fig. 2B).
The Mann–Whitney U test or Kruskal Wallis followed by Mann–Whitney U test were used to determine if differences between genotypes were significant (P < 0.05).
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However, the difference between genotypes was significant only for twin litters (P < 0.001).
In the threshold animal mixed model, the interaction between thresholds and genotypes was significant (P < 0.01) and the likelihood ratio test showed a better data fit when a separate set of thresholds was considered for each genotype ( and {τL+}), compared considering a common set of thresholds {τc} and an effect of genotype on the mean of the underlying variable.
There was also a genotype effect since differential expression between F2 and F252 genotypes was significant for NCED2, NCED5 and NCED6 genes (Fig. 5A, Additional file 4).
The post-hoc comparison between the Val/Val and Val/Met genotypes was significant (p = 0.04).
Our results found that the effects of soil moisture regime and genotype, as well as the interaction between soil moisture and genotype, were significant (p ≤ 0.05) for all physiological parameters (Table 3).
The variances of genotype were significant at P = 0.01, and the variance interactions between genotype and environment were not significant for the measured traits (Table 2).
Many metabolite differences between genotypes were statistically significant, but significance was not detected after application of a 20% false discovery rate.
For the male animals, those with the TT genotype received the highest score for odour, although again, the differences between genotypes were not significant.
For males the AA animals also had heavier sirloins although the GG animals had higher values of fat depth, however, differences between genotypes were not significant.
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