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In practice a protein-centric approach works well as proteins occupy a middle ground molecularly between gene and transcript information and higher levels of molecular and cellular structure and organization.
A significant correlation was found between gene and transcript levels, suggesting that MDM4 is a potential oncogene in BC with a p53-inhibiting function similar to that of MDM2 [ 25].
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Our results indicate a degree of concurrence between 3' gene and whole-transcript gene expression profiling: over 64% of the genes identified as significantly differentially expressed using 3' gene expression profiling methods are also identified through whole-transcript gene expression profiling.
We describe a detailed analysis of the similarities and differences between the gene and transcript annotation in the GENCODE and RefSeq genesets.
Although our method did not allow us to discriminate between different models of overall gene and transcript numbers in the cell, we believe it strongly suggests that more then 85% of transcripts are present in relatively low copy numbers (less then 100 copies per cell).
Gene descriptions and correspondences between gene, transcript and protein IDS were downloaded from Ensembl and Ensembl genome using XML-based queries using the Martservice utility of the BioMart (Haider et al. 2009) interface for each of the species.
This observation provided an explanation for the ambiguity observed in experiments between gene copy number and transcript status of MCF-7 and HeLa cells.
Sets of C. hamatus contigs mapping to the same subtree were further analyzed to discriminate between alternative transcripts of the same gene and transcripts from duplicated genes.
One reason for this may be that, in these taxa, such analyses are more difficult owing to the more complicated relationships between gene, transcript, and protein (for example, through alternative splicing), which blurs the definition of "homology" and makes meaningful comparisons among proteomes more difficult to achieve.
Despite very high similarity at the sequence level, the v.3 assembly annotation contains no history information that would provide links between contigs, genes, and transcripts in the second and the third versions of the assembly.
We have used RNA sequencing to identify differentially expressed genes and transcript isoforms between naïve males and males subjected to courtship-conditioning regimens that are sufficient for inducing long-term courtship suppression.
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Justyna Jupowicz-Kozak
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