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Finally, the relationship between fragment length and mean GC content was examined for five prevalent oral genera in dental calculus samples and a single prevalent soil genus in dentin samples to evaluate further the influence of genomic structure on microbial DNA survival.
To explore the CF to PAS distance (based on 3′ end of CF), the relationship between fragment length and proximity to the PAS was examined.
Since there is an inverse correlation between fragment length and amplification efficiency for ancient DNA, contamination from modern DNA could be identified by this assay.
Owing to the limited variation in DNA fragment size observed with shearing, the probability of generating starting fragments of the same lengths is high, leading to a nonlinear relationship between fragment length and clone size [ 22, 46].
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Analysis of the relationship between genomic GC content, DNA fragment GC content, and DNA fragment length reveals an inverse relationship between DNA fragment length and GC content in taxa with low- and medium-GC genomes, suggesting a systematic loss of short AT-rich fragments.
All methods exhibited a correlation between the fragment length and profile divergence.
In particular, array comparative genome hybridization and paired-end mapping, in which the presence of a CNV is suggested by a size difference between the fragment length and the corresponding region of the reference sequence (Korbel et al. 2007).
Correlation between fragment length polymorphism due to variable number of UGMS repeat-units in the functional domains and alteration of the predicted protein structure and active ligand binding sites suggested functional relevance of the genic microsatellites.
Finally, as a consequence of PCR primer pairs being positioned to optimize their amplification efficiency rather than to satisfy specific distance criteria from the embedded STR regions, the absence of a relationship between mean PCR fragment length and He accords with the view that PCR fragment lengths are not comparable in a meaningful way across microsatellites.
"Mate-inner-dist" (for paired-end data) was estimated by the difference between the middle RNA fragment length and twice the read length.
However, the maximum fragment length and sequence divergence between homeoloci where HRM remains useful for SNP or mutation detection is unknown and further experiments are required.
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