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Correlation between expression and grade and individual stainings were evaluated using Pearson chi-squared test for independent variables.
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As already previously reported in a small cohort of patient samples [ 25], high expression of ALDH1A1 was more often observed in well-differentiated tumours, though the correlation between expression and tumour grades did not reach statistical significance (Table 1).
In contrast, we observed negative correlations between HER4 expression and grade, recurrence and death.
In the present study, we found a significant association between DPD expression and grade in UTUC.
A significant negative correlation was found between EMA expression and grade (ρ = −0.356, P = 0.001).
A positive association between eIF4E expression and grade has been reported earlier (Li et al, 2002).
Spearman's correlation coefficient was used to evaluate correlation between protein expression and grade of dysplasia.
The Jonkheere Terpstra test was used to examine whether there was any association between marker expression and grade.
No correlation was found between RAD21 expression and grade, size, or lymph node, ER, or HER2 status (all P > 0.05).
No correlation was observed between MGMT expression and survival, either for the entire cohort or for each WHO histopathologic group but a correlation was observed between MGMT expression and grade classified as 'high-grade' (higher expression) vs 'low-grade' (P=0.0126).
Moinfar et al (2003) reported a higher rate of AR expression in especially low-grade DCIS as opposed to high-grade DCIS, although others did not find a correlation between AR expression and grade (Selim et al, 2002).
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