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Correlation between experiments was estimated using Pearson's correlation coefficient.
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In physiological optimization of experimental conditions, the variations between experiments were estimated by standard deviations (S.D).
The variations between experiments were estimated by standard deviations and the statistical significance of changes was estimated using the student's t-test.
A statistical correlation between in vivo experiments was estimated.
The strength of differential expression between any pair of experiments was estimated by M i = log2 ratio) = X i - X a, where a represented one particular cell type and i represented each given cell type in the set.
The strength of differential expression between any pair of experiments was estimated by M i = log2 ratio) = X i- X a, where a represented one particular cell type and i represented each given cell type in the set: B (basal), L (luminal), S (stromal), E (endothelial), G3 (Gleason 3) or G4 (Gleason 4).
Expression differences per protein spot between the spl5 mutant and WT from 3 independent experiments were estimated by t-test (p < 0.05).
For animal experiments, survival was estimated with Kaplan-Meier analysis.
As all studies that reported interviewer-rated symptoms used the Hamilton Rating Scale for Depression (HRSD), and as the HRSD is a widely used and well-validated measure of depression, this measure was chosen as the outcome measure for interviewer-rated symptoms, and the WMD between the control and experiment group was estimated at the follow-up points.
Heterogeneity between studies was estimated using the I2 statistic.
For a fair comparison between on-chip and off-chip experiments, the MMP activity rate was estimated from off-chip experiments in a manner similar to that of on-chip experiments.
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