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Moreover, sampling errors in NCP-cultured cells are not significant between experiments, because the spheroids formed in NCP are better in shape and size and can be easily observed microscopically.
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These quantities differ between experiments because different volumes of sample were processed (some samples were split for parallel analyses) but all samples analyzed satisfied mass balance (initial media Mo = final media Mo + cellular Mo).
This may be related to the difference in antigen loads between these two experiments because the TCR spectrum correlates negatively with the antigen load29 or constituents of the vaccine such as stabilisers.
Separate peaks from the heterodimer and mutant homodimer were not resolved in sedimentation experiments because the differences between the molecular weights and shapes of these species are small.
Large-scale metal-induced rearrangements would be masked in our crystal soaking experiments because the interfaces between YjiA molecules are already preformed in the crystal lattice.
A between-experiments ANOVA comparing the results of Experiments 1 and 2 (with Experiment and Load as between-subjects factors) further confirmed this, because no interaction effect on d' was found between perceptual load and experiment (F < 1); see Fig. 2.
Because some conditions differed between experiments, the six lines were not analysed together; separate ANOVA were performed to compare the lines from Experiment 1 (broiler chicken, layer chicken and Pekin commercial hybrid) and the lines from Experiment 2 (male Pekin line, female Pekin line and mallard).
In addition, the optimal intermediate wavelength was not easily predictable because it varied between experiments based on the relative concentration levels and expression patterns for each fluorophore.
We could not use GUVs for this experiment because the reported dissociation constants between 3B and 3D are in the tens of micromolar range and it is not possible to use micromolar lipid concentrations in the case of GUVs.
It demands an experiment, because the question is a comparison between one approach and another.
Because the only difference between Experiments 1 and 2 was the retention interval that preceded the final test, we also compared misinformation errors of commission on misleading trials (where that information was actually presented) across the two experiments.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com