Fragment cross sections show less agreement between experiments, and there are substantial differences between NUCFRG2 predictions and the data.
Figure 12 Comparison between experiments and simulations.
By setting up three experiments and three harvests, we ensured that all observed differences are reproducible between experiments, and not just between samples harvested in a single experiment, which is a commonly used strategy.
Although a significant difference in the overall activity levels was also observed between experiments (p<0.01), the interaction between experiments and infection was not significant (p = 0.82) indicating that the difference between infected and uninfected females was consistent.
When possible, a nested ANOVA method was used to calculate the variance between experiments and the variance between groups and to draw comparisons.
Example(s): The study in Box 1 has not been subject to a formal sample size calculation due to a lack of available data on the magnitude of the various components of variation planned into the design (e.g., the variation in flow rates between time-points in a particular experiment on a particular unit, the variation between experiments, and the variation between units).
An advantage of microinjection is that the amount of protein injected per cell is consistent between cells and between experiments and can be kept low relative to overexpression by transfection of cDNAs, which may better reflect the reported low levels of endogenous p110α and p85α [ 44].
Several methodologic issues present challenges to validation of oxidative stress biomarker assays, including highly variable background levels of specific DNA lesions (e.g., between individuals and between experiments) and the need to consider biomarkers of nitration as well as oxidation (Mayne 2003).
This technique provides a means to ensure reproducible sample pressures between different experiments, and even between very differently designed gas-flow stages.
As a result, FYPO supports sophisticated querying, computational analysis and comparison between different experiments and even between species.
