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Teaching Drosophila mating schemes provides an excellent means to enrich the experience of students on practical courses and fill the time gaps between experimental steps of a protocol.
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Workflow illustrating the experimental steps of the study is demonstrated in Figure 1.
LAV was involved in all experimental steps and drafting of the manuscript.
The experimental steps after the addition of gentamicin were carried out at ambient light.
It is well known that NGS data involve a number of experimental steps for sample preparation.
Since we are presenting a diversity of results, both experimental and analytical, to assist the reader in navigating our work, Figure 1 gives a graphical summary of our experimental steps, the results produced there from, and the connections between the different results, both experimental and analytical.
Refer to Figure 1A for a timing scheme of the experimental steps.
Current experiments address the variance between identically treated cultures and will furthermore allow an estimation of putative biased experimental steps.
The same experimental steps were repeated for the production of AC/f-CNSs (Scheme 1).
As acknowledged by Green et al. [7] and Rasmussen et al. [8], high-throughput sequencing studies of ancient genomes are not exempt from this problem, since the first experimental steps do not discriminate between endogenous and exogenous contaminating DNA molecules.
Samples were stripped of any identifier referring to fixation or processing method during all experimental steps.
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CEO of Professional Science Editing for Scientists @ prosciediting.com