The collector was placed between the two needles distanced 10 cm from each needle, and the polymer solution was ejected at 1 ml/h through a 21-gauge stainless steel flat-tip needle at room temperature and relative humidity at 30%%.
Therefore, the needles were used as electrodes and connected to the generator; in these conditions the depth of insertion was about 0.5 cm and the gap between the two needles was 0.9 cm.
Then, pull the yarn clockwise across the front of the right needle so that it sits between the two needles.
When you get to the part where the heel rejoins the main sock, pick up the yarn from between the two needles and make a new stitch.
c-Kit+ ECs were seeded using a bioreactor where the grafts were fixed between two needles and the bioreactor was set up in a standard incubator at 37 °C.
A constant 10 µA AC current was introduced at seven separate frequencies ranging from 1 Hz to 1 MHz to the outer two needles while the voltage was measured between the inner two needles.
For each set of four needles, a 3.3 kHz alternating (AC) constant amplitude current was introduced at three different amplitudes (20, 40, and 80 μAmps) to the outer two needles, while the voltage was measured between the inner two needles.
A 3.3 kHz AC current was then introduced at three different amplitudes (20, 40, and 80 μA) to the outer two needles while the voltage was measured between the inner two needles.
Needle number has been used to distinguish between the species, with P. monophylla having one needle per fascicle and P. edulis having two needles per fascicle.
