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In order to directly compare the fluorescence changes between embryos, we calculated the relative change in fluorescent recordings before versus during a muscle contraction (ΔF/F).
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For each experimental group of embryos, we calculated: i) the frequency of induction (and therefore recombination) within the group (number of embryos with recombination events/total number of embryos); and ii) the frequency of induction within the hindbrain from r2 to r6 (number of embryos with recombination events in the hindbrain/number of embryos).
Thus, in t = 3,4 embryos, we calculate that BicD levels are ∼0.267% of wild-type, i.e. have been reduced more than 300×.
To calculate dewlap color dissimilarity between species, we calculated pairwise Manhattan distances.
Note: we calculated distances between pairs of counties and pairs of metros based on housing-unit tract-weighted centroids.
Therefore, we calculated TC between all the Target fingerprints.
We calculated correlations between linked individuals to investigate mixing patterns.
We calculated divergence between the lineage-pairs using several metrics.
We calculated correlations between variables using Pearson's correlation.
We calculated distances between residences and major roads.
Second, we calculated the correlation between encoding and retrieval by leaving one electrode out (E[i]).
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