Adsorption and elution were densitometrically determined for BMP-2 by immunoblot analysis and for pln.D1 plasmid from agarose gel analysis, where biologic concentrations after TCP loading, then elution, were calculated from standard curves.
Specific elution was calculated by subtracting the fraction of complexes with buffer-only elution from the fraction eluted with dNTPs.
Elution times were calculated according to the elution profile of standard proteins of 1,400, 669, 150, and 66.4 kDa.
Molar masses spanning elution peaks were calculated using ASTRA v6.0.0.108 (Wyatt).
Their overloaded elution band profiles were calculated numerically using these isotherm data.
Overall yields, including elution yield and synthesis yield, were calculated corrected for decay in order to compare the labeling performance.
Approximate mass ranges of the proteins were calculated from elution times at half-peak height.
TPMP and MitoC standards were used to determine elution times and peak areas were calculated using the Chart 5 (ADInstruments®) software.
The molecular weights were calculated from the elution volume of polystyrene standards with molecular weight range of 5610 275,000 Da (Polysciences Asia Pacific, Inc. Taiwan).
The intensity ratios between heavy peptide standard and endogenous peptide were calculated based on the MS peak area at FWHM of the chromatographic elution profile of each peptide pair using the Xcalibur software (Thermo Scientific, San Jose, CA).
