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They were washed with PBST five times between each step of ELISA.
Plates were washed five times with PBS 0.05% Tween 20 between each step of the assay.
To determine the significance of and the relationship between each step of the degradative pathway, researchers have performed various inhibition studies.
Cells were then blocked with 10% FBS in DPBS (FBS blocking solution) for 30 minutes, and then treated 15 minutes with avidin/biotin blocker (Vector Laboratories, Burlingame, CA) with a DPBS rinse between each step of the procedure described by the manufacturer.
Between each step of staining the specimens were washed in distilled water or DPBS.
Three washes with PBS were applied between each step of antibody incubation.
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The six guidelines are based on limited evidence and major differences exist between them at each step of the uDCD process.
The ownership of an instrument should be defined in the beginning between all parties involved, and each step of the instrument's life, including distribution, should be anticipated for purpose of copyright.
It was assumed that some fractions of chain segments between entanglements were gradually broken in each step of stretching operation, rather than disentangled and slipped.
The CT was performed to evaluate the correlation of collapsed tissue amount in each step of PEEP between CT and EIT.
These target predictions were made by detecting miRNAs with significant expression changes at each step of differentiation between GPs and OLs.
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