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Between each step rinse the gel in distilled water.
All immunoreagents were diluted in CWFSG and the grids washed in PBS between each step.
Between each step, sections were washed with PBS, 0.1% Triton-X100.
Cells were washed with 1× PBS between each step, except for "blocking-primary antibody step".
Sections were washed three times in PBS between each step in the immunodetection protocol.
The temperature ramp speed between each step was 1 °C/second.
Between each step, the glass slide was dried with nitrogen.
A linear artifact is apparent between each step.
Six breaths between each step.
Washing occurred between each step.
Several rinses with 0.02M KPBS were performed between each step.
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