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The distance between each sampling point was within 100 m, and the ecological environment was consistent.
Total soluble solids, as measured by °Bx, increased consistently between each sampling point, with the most rapid increase occurring between weeks 10 and 11.
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There were differences in growth of the fish between treatments at each sampling point, but this was not related to the concentration of the effluents.
We assessed the extent of fecal contamination at the source and in household stored water (HSW) and explored the relationship between contamination at each sampling point and water supply type.
Differences between control and test at each sampling point were assessed with Student's t-test.
A two-sided t-test was used to establish whether the average concentration of each quantified metabolite was different between the two strains at each sampling point.
A forest map at the scale of 1 25,000 was used to extract information about vegetation for the area between 1000 and 5000 m from each sampling point.
ACHR was done using the COBRA tool box [43] selecting 19000 randomly distributed points with 1000 iterations between each sampled point.
Our approach characterizes the manifold structure of each sample point using the geodesic distances between the sample point and given correspondences.
Intervals between each water sampling point in the river were ∼1 km.
Overall significance levels for each covariate were calculated using Type III tests of the fixed effects of the interaction between sampling point (source or HSW) and the covariate of interest.
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