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For each pooled sample a linear regression and correlation analysis was also performed between each replicate at each RNA input level and one of the two replicates run at 250 ng of RNA.
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If, for each replicate, we look at the average posterior SPR distance per segment (actually, the distance between each segment and the next), we will have the distribution of the mean distances for each segment for 100 replicates.
In order to have a uniform cell population between replicates at each instance, the muscle cell homogenates from different mice were mixed well and the resulting cell mixture was used to prepare the cultures.
Enriched isotope CH3Hg+ tracers were present in amphipods after 12 h, and concentrations of both tracers in amphipods did not vary significantly across time series (Kruskal Wallis, d.f. = 3, p > 0.05), although variance between replicates at each time point was large (16 46%).
Each replicate was separated by at least 50 m.
In other words, each pairwise test between lines was replicated (at least 10 times), and each replicate was run on a randomly chosen day with flies of random age.
Furthermore, the threshold cycle (Ct) spread between the replicates at this concentration had to be less than one cycle.
Differentially expressed/present proteins following apoptosis induction were defined by boundaries representing 95% confidence intervals (CI) by assessment of variance between biological replicates at three different time-points.
Larvae grown at the standard (lower) temperature were pooled between culture replicates at 5 d post-fertilization and those pooled larvae used in short-term treatments expected to affect gene expression.
Controlled analysis done by limiting the effect of input sequence data on overall measurement of stochastics showed variability between technical replicates at the genotypeability threshold of ≥ 20x and this is addressed by aggregating data to higher depths.
After correcting for control mortality, the sterility of A. ferus adults ranged from 90.5 to 99.1% at 20 Gy and between 99.0 and 99.6% between the three replicates at treatment doses of 40 to 100 Gy.
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