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Variation between duplicates was set to a maximum of 15%.
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Two biological duplicates were set up, including four technological repeats in each duplicate.
All samples were performed in duplicate and variation between duplicates was <10% for every reported value.
The variability between duplicates was between 0 and 4.8%.
If the CV between duplicates was >15%, samples were re-analysed in duplicate.
Each reaction was performed in duplicate, and a ΔC (t) < 0.5 between duplicates was required.
Each culture was set up in duplicate.
Each assay was set up at least in duplicate.
Duplicate options were set for 'On-high similarity'.
We run other reactions only in duplicate as the differences between duplicates were less than 15%.
All conditions were set by duplicate.
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