The direct product of dose, density, and volume forms a quantity called integral dose.
Grade 3/4 haematological toxicities resolved appropriately, and planned dose density and dose intensity of RICER were preserved.
The statistical interaction between dose and density was marginally significant for day of death (H = 7.813, df = 3, p = 0.05; Figure 1B) with tadpoles exposed to Dose 3 dying significantly earlier in low density tanks but not when held in high density conditions (Figure 1B, Table S1).
While the statistical interaction between dose and density is not significant (F = 1.763, df = 3, p = 0.153, Table S1) it is worth noticing that control tadpole in low density tanks reached a significantly more advanced stage of development than infected larvae (F = 4.252, df = 3, p = 0.006, stage 34 for control vs. 32 for dose 1, 32 for dose 2 and 30 for dose 3, Figure 1C).
In the untreated group, no relationship between dose delivered or dose density was seen.
The epirubicin RDI was not different between treatment arms (P=0.87), although the dose density was twice in the FEC100 arm.
Increased dose density is achieved by reducing the interval between each dose of chemotherapy.
This is supported by data showing an inverse relationship between dose exposure and bone mineral density [ 7].
From this experiment, it was determined that the regression coefficients that had the most statistical significance were the type of dye (p = 0), current density (p = 0), and the interaction between current density and coagulant dose (p = 0.071).
In the low density tanks the tadpoles with the lowest growth rate were those exposed to the highest virus dose (Figure 1D) indicating a dose response at this density: there were significant differences between Dose 3 and control, and Dose 2 and control (F = 14.64, df = 1, p<0.001 and F = 6.07, df = 1, p = 0.0141, respectively).
