In fact, two biologics made using different cell lines and differing manufacturing processes will rarely, if ever, be exactly the same, hence the name "biosimilar".
When the data were stratified by sex, correlation was detected between differing cell types in males at CpG -186 (r = 0.42, P = -0.02) but not CpG -54 (r = 0.04, P = 0.84).
Here we have shown that metabolic profiling of cancer cells can not only identify phenotypic differences between cell lines but can also sensitively detect and distinguish changes in metabolite concentrations induced by relatively short exposure to differing drugs.
Overall cytoxicity varied between extracts and between cell lines.
The EC50 and EC90 values were also determined in LNCaP cells, a prostate cancer cell line, and there was a consistent difference of up to 5 fold between the two cell lines, which may be related to their differing rates of nucleoside uptake and bioconversion to the active nucleoside triphosphate analog.
To define variations in cell-cycle requirements between cultured cell lines, we generated knockouts across cell lines of diverse origins.
The overall antiviral activity of the Pre-SP, Post-SP, and Semen treatments exhibited similar trends between the differing cell densities.
However, there was no correlation in methylation between the differing cell types at CpG -54 (r = -0.03, P = 0.78).
Fold changes between cell lines are indicated.
Interestingly, therefore, our systems medicine approach was better able to explain the differing responses of the cell lines to TMZ than analysis of the methylation status of the MGMT promoter (predictive rates of 91 versus 73%, respectively).
