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The compatibility of the output VCF files between different methods as well as the NIST-GIAB gold standard was examined using bcbio.variation tools and manual inspection.
Note that, according to our experiments, the size of candidate set does not have a significant effect on the performance difference between different methods as long as it is greater than 20 (data not shown).
This approach strengthens the comparison between different methods as it eliminates any sampling variability leaving all methods striving for exactly the same results, whilst allowing variability to exist between simulated datasets and amounts of missingness.
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Know the differences between different methods of ingestion.
The p value between different methods are plotted as bridge and stars, where two stars means p < 0.05 after both Bonferroni and FDR corrections, and one star means p < 0.05 only after FDR correction.
Lack of agreement between different methods is inevitable, as all instruments measure with some error, but the questions of interest is by how much do the methods disagree and is this difference important?
It is probable that the angiosperm-wide survey of the patterns of evolution of these genes will identify the source of incongruence between different methods of the analysis and, as the result, improve their utility for plant phylogenetics.
Important differences were observed between different methods.
The ICC was chosen as an assessment of the agreement between different methods used on the same set of subjects.
First, as pointed out by Wu and Zhang [19], the threading performance is usually sensitive to the template library which varies greatly between different methods.
The most commonly used methods to calculate daily fluid balance, such as monitoring changes in body weight, are obviously imprecise with pronounced diversity between different methods [ 2].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com