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Protein expression of bPAC in testis lysates varied between different founder lines.
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Two different founder lines were established.
Using this information, we have developed a PCR-genotyping method that allows these three different founder lines of MMTV-Cre mice to be readily distinguished.
Importantly, tumor nodules from multiple C/T790M mice from different founder lines do not appear to harbor secondary kinase domain mutations arising in cis.
Using a PCR-based genotyping method, the three different founder lines can be distinguished, and we determined that the MMTV-Cre line A, the most widely used MMTV-Cre founder line, exhibits a profound lactation defect that limits its use in studies on mammary gland development.
(C ) Western blot analyzing bPAC-HA expression in testis lysates from different founder lines.
We report here on our methodology and present some preliminary characterization of different founder lines towards advancing this technology.
As identical expression patterns and phenotypes were observed in females from the different founder lines, data presented here were obtained primarily from the pW224-62 Tg line.
A different founder line (A_K15CreERlow) exhibited low expression levels of the transgene and individual cells could be targeted by Tam administration.
While fewer introduced individuals may have led to reduced diversity in the parasite, admixture between different founder populations may have counteracted genetic drift and bottlenecks.
Similar results were obtained for three different Deletor founder lines (26) (not shown), demonstrating that the observed effect was not a consequence of the transgene insertion sites.
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