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Laboratory confirmation delay (time between date of symptom onset and date of laboratory confirmation) was significantly shorter in 2009 compared to 2008 (p = 0.0001), with a median delay of 20 days in 2009 compared to 29 days in 2008.
Duration of illness was identified by discussion with the case-patient or, if the case-patient could not be contacted, was defined as the difference between date of symptom onset according to the medical record and date of hospital discharge.
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The clinical serial interval was the number of days between dates of symptom onset of successive cases linked in a transmission chain.
Duration of survival was calculated as time between date of first symptom attributable to PCNSL and date of death or January 1st 2008.
Patients were considered not vaccinated if time between date of vaccination and symptom onset was <14 days.
The lag time between coded date and date of symptom onset (as manually validated) ranged from 1 day before to more than 60 days before the automatically detected event date.
In addition, we explored the delay between date of birth, date of first symptoms (FS), date of diagnosis, and date of therapy or death.
The date of infection was estimated on the basis of one of the following: the date of symptom onset minus 15 days, the date of the incomplete Western Blot minus one month, or the mid-point between a negative and a positive ELISA test.
Clinical variables recorded included date of symptom onset and date of death (disease duration calculated as the difference between these), presenting symptoms, clinical dementia, and family history of ALS.
The date of HIV infection was estimated as the date of symptom onset minus 15 days, the date of an incomplete western blot minus 1 month, or the midpoint between a negative and a positive ELISA.
Date of symptom onset was not recorded for 32 patients.
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