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Normalization between datasets was done with uniquely aligned spike-in RNA reads.
Statistical evaluation of phylogenetic signals between datasets was done using SH tests [ 58].
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Each dataset was done in replicate.
Dataset correlation was done through MiRGator v3 software [ 77].
For the Affymetrix dataset, results were compared between the dataset normalized with MAS 5.0 alone and MAS 5.0 + NEPS scaling and for the Agilent dataset, the comparison was done between median normalization alone and NEPS scaling followed by median normalization.
Only the markers overlapping between the source datasets were used; no imputation was done.
This analysis was done for all datasets between the 8. and the 11.
The calculation of MAPE was done for the testing dataset and the training dataset.
This was done for the whole mitogenome dataset and for each sub-dataset containing variable sites.
This exercise was done using the training dataset.
The verification was done for totally new 8 datasets.
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