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Future studies can investigate the significance of the differences between crypt epithelial cells and myofibroblasts in expression of these TLRs.
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To further test if the microarray analysis accurately represented the differences in mRNA levels between villus and crypt epithelial cells, we evaluated expression of four genes by RT-PCR using the cDNA pools obtained by LCMD.
To provide further support that the transcriptional profiles detected by microarray analysis accurately represented the differences between villus and crypt epithelial cells, we evaluated expression of selected genes by immunohistochemistry.
A minimum 2-fold difference between mean levels of transcription in villus and crypt epithelial cells was used as criteria for determining "enriched" expression in each population.
Cellular composition of crypts was quantified by counting the number of crypt epithelial cells and Paneth cells (Fig. 6B).
EPEC entered 10% of colonic crypts and adhered to crypt epithelial cells, often in the proliferative compartment.
Since ionizing radiation induces apoptosis of intestinal crypt epithelial cells, we performed TUNEL assay to examine apoptosis of crypt epithelial cells, 1 day after WBI.
Crypt epithelial cells proliferate rapidly and are highly sensitive to cytotoxic agents and irradiation.
The renewal of crypt epithelial cells largely depends on a sub-population of stem cell progenitors residing at the base of crypts.
Two h after the i.v. injection, BrdU immunoreactivity was exclusively found in the crypt epithelial cells (Figure 3).
We predicted that levels of phospho-Tyr-15 would be elevated in crypt epithelial cells lacking the CDC25s.
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