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Whereas genes with similar signal in either channel are expected to have LRs near zero, genes with LRs that deviate significantly from LR = 0 are likely to show copy number changes or sequence divergence between control and tester strains.
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The adapter-linked control and tester DNA are reassociated through mixing at a ratio of 1 1, denaturing and annealing.
The PCR not only exponentially amplifies the control and tester DNA but also separates them from each other.
In short, the cDNAs from driver (control) and tester (As-treated) samples were digested with endonuclease RsaI and the tester cDNAs were ligated to two different adapters at either end of the cDNAs separately [ 52].
Briefly, the control and tester cDNA's are digested with Taq I restriction enzyme, then ligated with the CT and TT adapters, respectively.
The DNA reassociation results in the quadratic magnification of expression ratios for the up- and down-regulated genes in control and tester samples.
The LR distribution of genes with 100% identity between NCTC 11168 and RM1221 (n = 114) would be expected to behave much like that of self-self experiments because in both cases Control and Tester targets are identical and thus have 100% PTI.
With either approach, control- and tester-derived targets are co-hybridized to the microarray and control- and tester-derived signals are compared, often by computing the Log Ratio (LR) = log2 tester signal/control signal).
Inter-observer reliability between trainer and tester with 20 mothers was high (r = 0.98).
This study investigates alignment between developers and testers in software development to understand alignment within the IT unit.
Briefly, ΔCT values were obtained and ΔΔCT values (fold differences between reference and testers) were calculated.
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