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We can better interpret the relationship between contigs by using mate-pair reads.
Every contig was compared to every other contig using BLASTN.
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To assay the assembly quality, all the PE reads were aligned back to these contigs by using Bowtie2 program (v2.0.0-beta5 v2.0.0-beta5 57gned rand was calculathe.
For example, analyses of BAC end sequences and preliminary generation of BAC contigs by using the above libraries have been conducted.
Gaps between contigs were closed by using the Consed editing software [213], custom primer walks, and/or PCR amplification.
The relationship between contigs was displayed using ContigScape [ 84], determined by sequence alignment analysis and verified by PCR.
Connections between these contigs and adjacent contigs with similarly high coverage were identified by looking for sequencing reads that were split between contigs by the assembly software.
Plasmid analysis of the Bf contigs was performed by using BLASTN with the pCLJ sequence and 70 Bf contigs.
It extends contig ends iteratively by using reads overlapping with the contig end.
Our results demonstrate that a 630-kb region can be rapidly cloned and mapped into contigs by use of the BAC system.
The reference-ordered and -oriented genomic scaffold that was used for the subsequent analysis was generated by concatenating reordered contigs by inserting 5Ns between the contigs using an in-house python script.
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