Intriguingly, modified eBURST analysis revealed in both mice several connections between single cell clones derived from distant tissues (such as from contralateral tissues), suggesting that cell migration may occur during development, such that spatially separated cells share similar mutation profiles.
We have assessed the quality of the amplified products generated from 10 pg input DNA using high-throughput sequencing of DNA extracted from 17 progeny clones derived from genetic crosses between two laboratory strains.
These limitations relate primarily to several forms of variability arising from differences between hiPSC-neurons derived from a single patient, from independent clones derived from a single patient and from differences between hiPSC lines derived from different patients.
We did not detect significant differences between biological replicates, i.e. different iPSC clones derived from the same patient or control, tested in parallel for selected experiments (supplementary material Fig. S17).
Haploid progeny parasite clones derived from a genetic cross between two parent clones (HB3 × Dd2) with distinct drug-selection histories was central to mapping the molecular determinant of chloroquine (CQ) resistance [ 56] and several other complex trait loci [ 57- 64].
We observed a greater degree of similarity between the two groups of SRS clones (hypomethylated versus normomethylated) than between each of these groups and the group of control clones derived from healthy patients.
Multiple cDNA clones derived from Riken libraries map to the region between Irm and Meg9.
Nucleotide sequences used in this study were selected from a sample set of 1,562 HIV-1 env V1-V3 clones derived from 156 blood plasma samples of 82 individuals, collected between 1993 and 2008 (Table S1).
All iPSC clones derived from FRDA patients retained pathological GAA repeat expansions, ranging between 400 and 1000 triplets (Fig. 1A), with the exception of one clone, in which one allele contracted to 200 GAA triplets, a size still within the pathological range FD135-4LL clone 28; Fig. 1A).
The most recent "Star Wars" movie, last year's "Attack of the Clones," featured an army of clones derived from the genes of an aggressive bounty hunter, modified to ensure willingness to follow military orders.
