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The title is something like a mix between Clash Of Clans, Farmville and a racing game.
Overlap between CLASH Targets and Experimentally Validated miRNA Targets from Published Databases To check if our CLASH data set contains known, experimentally validated miRNA targets we compared it with two databases – miRTarBase (Hsu et al., 2011 ) and TarBase (Vergoulis et al., 2012 ).
To calculate the overlap between CLASH targets and high-confidence AGO-binding sites obtained in the PAR-CLIP study we first mapped the sequences of the 17,319 clusters from Table S4 in (Hafner et al., 2010a ) to our transcriptome database.
To calculate the overlap between CLASH targets and bioinformatically predicted miRNA targets by miRanda (John et al., 2004 ), TargetScan (Lewis et al., 2005 ), PITA (Kertesz et al., 2007 ), PicTar (Krek et al., 2005 ) and RNAhybrid (Rehmsmeier et al., 2004 ), we extracted the coordinates of predicted miRNA target sites in the hg18 genome from the Functional RNA Project website (www.ncrna.org ).org
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com