However, the relationship between nucleosome position and gene expression is complex, and there is not always a correlation between changes in nucleosome occupancy and transcriptional activity of a gene.
This study identified the tup1 and cyc8 single deletion mutants as having a particularly strong relationship between changes in nucleosome occupancy in the − 200 to transcription start site (TSS) region and the expression of their target genes [53].
There was significant interaction between change in vegetation and site occupancy in the first year in the analysis of all males.
We show that a dramatic reduction in occupancy after clear-felling was not paralleled by a similar change in occupancy at sites used as controls, where occupancy was stable through time.
The rest of secondary proteins are in the moderately affected group, with up to 2-fold change in occupancy.
In Drosophila, we observed changes in turnover with no net change in occupancy in gene bodies (PMID: 2208596).
Three physical properties that we defined, the repositioned variation, the occupancy change and the positioning degree change, were used to characterize three major types of differential nucleosome positioning: changes in nucleosome location, changes in occupancy and changes in fuzziness.
There was no significant change in Rsc2 occupancy; however, Rsc1 occupancy was decreased by approximately forty percent in the rco1Δ mutant compared to the wild type strain.
These results reveal that change in nucleosome occupancy between in vivo and in vitro not only occur around TFBSs, but also in broader regions at vitro+/vivo- and vitro-/vivo+ promoters.
Figure 4 Change in oxygen occupancy (∆g O ) analyzed from the Rietveld refinement, MEM, and XPS studies.
