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A simple explanation for the difference between cells lines with respect to mTORC2 inhibition by rapamycin could be impairment in mTOR's intrinsic negative feedback loop, described above.
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We examined 7 of the 270 genes by quantitative PCR and validated that 4 (57%) were differentially expressed between cell lines with L858R mutations versus those with Δ746 750 mutations (File S6).
There are marked differences in the relative expression of these host receptors between cell lines, with Detroit 562 (nasopharyngeal) cells expressing higher levels of pIgR than A549 (type II pneumocyte) cells, while the reverse is true for the PAF receptor [34], [35].
The differences observed between cell lines with respect to H3K9me2 are consistent with the highly contextual dependence of LSD1 function.
In principle, TD-GC MS resulTD-GC MSthat theresultsdetectable VOC differenceshowthatn cell lines witherenimaregenetic detectables.
The strategy then incorporates epigenetic regulatory patterns that differ between cell lines with disease-specific transcript alterations in t-AML.
The extent of decrease varied between cell lines with a significant decrease observed in A2780, SKOV-3 and OVACAR-3.
Furthermore, we found no significant differentially expressed genes between cell lines with wild-type and mutated CDH1.
Expression was significantly different between cell lines with 5p amplification, cell lines with 5p gains or no 5p alterations, and NPE.
Real-time RT PCR revealed that expression levels of both receptors differed between cell lines, with approximately 2-fold higher BLT1 mRNA expression compared with BLT2 expression (P<0.001).
It selected eleven genes that were differently expressed and which had different promoter methylation patterns between cell lines with and without metastasis ability.
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