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The majority of gene expression differences were observed between cells captured from both intraductal components pure DCIS and the in situ component of DCIS-IBC.
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Two types of stem cells being investigated are embryonic stem cells (ESCs, captured from embryonic mammalian cells) and induced pluripotent stem cells (iPSCs).
A total of 100 500 cells were captured from each section and cells from two adjacent sections were pooled.
From the total pool of normal ovarian tissues, approximately 10,000 cells were captured from cystic inclusions and 10,000 cells were captured from the surface epithelium; a similar number of cells was obtained from the ovarian carcinomas.
On average, 84 cells were captured from each micrograph; between 900,000 and 2.4 million cells were segmented from each experiment, translating to more than 13 billion numerical cell-level image measurements, which were stored in the database.
Values were obtained from each cells captured in five high power fields (× 400).
Approximately 3000 tumour cells were captured from each specimen for analysis.
Approximately 500 cells were captured from each lesion and collected onto a dedicated cap.
c The relationship between cell capture efficiency and cell density.
b The relationship between cell capture efficiency and cell trapping time.
a The relationship between cell capture efficiency and side length of square microwells.
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