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Extending this idea, it has been debated that it is not synergy itself that is pharmacologically useful, but differential drug interactions between cell types, with the essential goal being a more favorable interaction on the target cell type than on non-target cell types15,16,17.
This allowed calculating mutual overlaps between cell types with higher precision, when compared with the overlap of their somata (Supplementary Fig. S3 and Table S2).
Strikingly different protein bands are observed for the same cell type with different antibodies and between cell types with the same antibody.
In contrast, differences in frequency of spontaneous excitatory synaptic inputs were more prominent between cell types, with D2 cells receiving significantly more excitatory inputs than D1 cells, particularly in the VMS.
Only GFR showed a significant difference between cell types, with endothelial progenitor cells (EPCs) causing significantly more improvement in GFR than other cell types (P<0.001, supplementary material Fig. S4C).
We found that Cx43 expression levels differed between cell types, with MCF10A and BT474 cells expressing low levels of Cx43 in comparison to MCF7 and MDA MB 231, which showed higher expression levels of Cx43.
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These alternate routes to signaling are further modulated by the fact that the complement of signaling partners will not only differ between cell types but also with respect to subcellular localization.
Hence, it might not be surprising that the molecular sequel of aging varies between different cell types with different regulatory networks, different environments and different cellular fates.
Changes in the expression of the 11 candidate genes widely varied between different cell types with the direction of change of expression being randomly different from what we observed in microarray hybridizations (Table 1).
We did not find striking differences between both cell types with respect to influence of coculture on collagen gene expression and cytokine, bFGF and GAG release.
The dose-response profile obtained showed a difference in sensitivity between the cell types, with neurons showing considerably less sensitivity to mTHPC-mediated PDT than the other cell types.
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