Between the bud and leaf, the leaf and berry, and the berry and bud assemblies, 12,122, 12,298, and 23,033 unigenes were shared, out of a total of 100,237, 94,093, and 139,364 unique transcript sequences from each pairwise comparison.
For the "bud and leaf" type, we combined the data for "one and a bud" and "two and a bud".
All of these bud and leaf tissues shared 50,499 unigenes, which likely correspond to genes that provide essential functions in buds and leaves (Fig. 4b).
A distinction is made between bud and apical meristem cultures.
Finally, if we test for expression differences between the leaf RNA pool and each of the bud RNA pools (bud and bud'), many genes were found to be consistently differentially expressed between bud vs. leaf and bud' vs. leaf, as seen by the high correlation among the most significant p-values.
Adults were observed feeding on soft tissues of flower buds and leaf axils.
Mature six-month-old plants of the same hybrid were used to obtain lateral buds and leaf roll (apical meristem plus leaf primordia) tissues.
As the snow melts, I track bud break and leaf development.
The last two samples collected corresponded to emergence of pubescent bud scales and leaf emergence, respectively.
The expression levels in stem and immature seed were much higher than those in root, trifoliate leaf, flower bud, pod and leaf.
