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The accuracy of the spatial correspondence between brain sections and MRI slices was about 0.5 mm.
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In situ hybridization using nurse bee, forager, queen and drone brain sections and Amfutsch-specific antisense RNA probes revealed a similar expression pattern in the brain between all of the above four bee types.
The hypothesis arose through the input of researchers with a history of observing prion particles [ 4, 5] seeing parallels between these entities in brain sections in Creutzfeld-Jacob disease and the plaques in AD brain, described years earlier [ 6].
Brain sections were prepared and examined quantitatively.
3 mm coronal brain sections were made and embedded in paraffin wax.
Brain sections were coverslipped and incubated overnight at 55°C.
Brain sections were collected and stained as described above.
Brain sections were then thaw-mounted on poly-l-lysin-coated glass-slides and stored at −55 °C for immunohistochemistry. Additionally, brain sections between the vascular organ of the lamina terminalis and the median eminence (bregma 0.6 to −2.8) were stacked on glass-slides, and the hypothalamus was dissected.
f, Representative images of somatosensory cortical brain sections (acquired between layers IV and V) from P0 Dcx−/Y chimaeras co-stained with Dcx (red) and GABA (green) antibodies.
g, Representative images of somatosensory cortical brain sections (acquired between layers IV and V) from P0 Dcx−/Y chimaeras co-stained with Dcx (red) and GAD67 (green) antibodies.
b Staining differences between WT and S1R-KO brain sections were calculated by comparing pixel intensities of regions of interest after normalizing the sections to their background reading.
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