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Comparative hybridizations were conducted in a circuit design, permitting comparison between both isolates at all three life-cycle phases, and among all life-cycle phases for each isolate.
Similar(59)
No growth was observed for both isolates at aw 0.801, growth only occurring at 25 and 30 °C at aw 0.822.
This is deadlock at touching area type of interaction A. terreus RPW1/6 A. alternata RZWM 3/2 A very prominent clear area was observed between the both isolates.
The high mapping percentage suggests a high similarity between these isolates, at least at the transcriptome level.
Mean (±SE) difference ingermination rate between Southern and Northern isolates at 10 and 20°C, as measured in the main experiment (dark grey circles) and additional experiment (light grey circles).
However, the unmatched number of 24 complete Cpn genome sequences allowed us to investigate the evolutionary relationships between Cpn isolates at a novel resolution level.
As previously reported (3 ), despite significant variability between TOSV isolates at the nucleotide level (up to 13.2%), phylogenetic analysis of amino acid sequences did not allow discrimination between the Italian and Spanish genotypes (Appendix Figure, Figure 2B).
Consequently, it is possible to consider the (potentially empty) set of genomic sites that have not been affected by recombination since a sample of isolates evolved from a common ancestor, and the ancestral relationships between the isolates at these sites is called the clonal genealogy (Guttman 1997).
The 16S rRNA gene sequences allowed separation between isolates at the species level.
This annotation file can be opened with the Blast2GO software and by uploading each list of strain-specific varying genes, Blast2GO functions can be used to interrogate data for significant functional differences between isolates at the genome level, and to obtain the functional profiling of the genomic alterations or to locate mutated genes in metabolic pathways.
Table 2 indicates possible transferases that could be causing the phenotypical changes of the goblet cell mucins seen between the two isolates at d7 p.i from the whole gut microarray analyses.
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