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Because we observed a bigger difference between both analyses for mice, we additionally performed an intermediate 30 component analysis.
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A clear correlation was observed between the expression values of both analyses for all three genes.
All together, validation of microarray data by qPCR revealed a high correspondence between both analyses, confirming differential expression for 19 out of 25 genes tested (p < 0.05).
However, the sample size limited between-group analyses for both race and ethnicity.
Pairwise FST values increased significantly for all comparisons between populations in both analyses except for PS vs. PHA and PS vs. PHG for which FST values decreased non significantly (P = 0.073) from 0.019 to 0.014 for PS vs. PHA and from 0.022 to 0.015 for PS vs. PHG (Tables 5 and 6).
We adjusted all subsequent between-group analyses for identified appreciable differences.
If potentially confounding imbalances are found, we will adjust the between-group analyses for potential confounders of treatment assignment.
For PXO99 (race 6), pronounced differences in the Manhattan plots were found between association analyses for the indica/aus panel and the indica subset.
There were no statistically significant differences for either within or between group analyses for QoL.
These findings are also reported in Table 3 (panel B) and suggest little differences between the analyses for all three groups combined.
For between-person analyses, results for each analyte were filtered to include one pre-intervention result per subject and were sub-grouped by gender.
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