- Our recent survey of 61 (17 B biotype and 44 Q biotype) field populations collected from different plant species and locations in China has demonstrated that at least three factors including biotype or genetic group, host plant and geographical location affect the infection dynamics of the S-symbionts in B. tabaci.
The B biotype population was first collected from cabbage, Brassica oleracea var.
With the exception of the Df field, aphids of different biotype collected at the same site did not show significant genetic differentiation.
The wheat crown tissue (one-cm segments from leaf sheath 2) was collected from both infested (biotype L infested H9-Iris and Newton) and uninfested control plants over a time course (4, 6, and 8 DAH) as described previously.
For the observations on fecundity and longevity of non-viruliferous and viruliferous adults in each of the two biotypes, approximately 200 newly emerged adult whiteflies were collected from the culture on cotton and divided randomly into three groups of 60 70 each, to be used for inoculation onto the three types of tobacco plants: healthy, TbCSV-infected or TYLCCNV-infected.
To prepare cDNA libraries for RNA-Seq and to perform subsequent qRT-PCR validation, A. glycines were obtained from a biotype 1 laboratory colony that originated from insects collected from Urbana, IL (40° 06′ N, 88° 12′ W) in 2000 [ 67].
The larva was collected from shallow water between Florida and Cuba.
For each biotype, approximately 300 newly emerged (0 24 h) adult whiteflies were collected from the culture on cotton and divided randomly into three groups of about 100 each, to be used for inoculation onto the three types of tobacco plants: healthy, TbCSV-infected or TYLCCNV-infected.
Three of the isolates (all toxigenic and of the mitis biotype) had a unique RT not previously described; they were collected from 1977 through 1981.
The tweets were collected from over 167 weeks between November 2011 through December 2014.
