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Hence we compared CN from sequencing to the median intensity signal of the probes covering the region from array platforms as shown in Figure 5B and C. We find a high concordance between array platforms and WaveCNV.
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Data were cross-validated between array platforms using GSEA and the method of Cheadle et al (2006) [32].
We examined the correlation of the log ratios between the array platforms and miRNA expression measured using TaqMan arrays, as qRT-PCR is often considered to be the 'gold standard' for validation.
Furthermore, this meta-analysis can be accomplished simply by using UniGene and RefSeq identifiers as common variables between array platforms, though UniGene is shown to be slightly better at achieving this discrimination in our dataset.
The logratio values are compared between array platforms in Figure 2. Gene selection was performed for the toxicology and platform comparison projects using different criteria, although both criteria are based on the expressional differences and noise levels in measurements.
This method was developed to examine the entire dataset, taking into account the differences in absolute mRNA quantitation which often occur between array platforms.
This was in accordance with a previous study performed by Johnson et al. showing that the variation in performance between labs, protocols, and algorithms within the same array platform was greater than the variation in performance between array platforms [22].
Probe mapping between array platforms was done based on Entrez Gene IDs.
Data regarding batch variability should also be recorded and reported Common reference RNA standards (housekeeping genes) should be adopted to facilitate comparison between array platforms.
The exception in these clusters is gene 7-4, which displayed inconsistent light inducibility between the two array platforms and low absolute expression according to the NSF45K array; it is excluded from the following analysis.
This was performed by determining the difference in genomic length for each location between the two array platforms and then selecting the minimal distance.
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